Surface ozone variability at Pune (an urban location) and nearby rural locations has been studied using KI solution chemical method. The measurement showed well-marked diurnal cycle of ozone concentration with minimum at sunrise and maximum at noon hours. Simultaneous measurements of humidity and temperature along with ozone suggest that the ozone concentration is directly proportional to temperature and inversely proportional to humidity. The averaged diurnal variation of ozone during different months and two seasons, viz. winter and spring show high ozone concentration over the rural locations than the urban location. Higher ozone concentration at the rural locations may be due to slower titration of ozone by nitric oxide in the evening hours.