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Home > Featured Articles > Diverse antibiotic resistance genes in dairy cow manure > Diverse antibiotic resistance genes in dairy cow manure

Diverse antibiotic resistance genes in dairy cow manure [1]

Application of manure from antibiotic-treated animals to crops facilitates the dissemination of antibiotic resistance determinants into the environment. However, our knowledge of the identity, diversity, and patterns of distribution of these antibiotic resistance determinants remains limited. We used a new combination of methods to examine the resistome of dairy cow manure, a common soil amendment. Metagenomic libraries constructed with DNA extracted from manure were screened for resistance to beta-lactams, phenicols, aminoglycosides, and tetracyclines. Functional screening of fosmid and small-insert libraries identified 80 different antibiotic resistance genes whose deduced protein sequences were on average 50 to 60% identical to sequences deposited in GenBank.

Original Source [2]

Publication Date: 
22/04/2014
Fabienne Wichmann [3], Nikolina Udikovic-Kolic [4], Sheila Andrew & et al [5]
mBio [6]
5
2
Tags:
Animal Dung [7], Micro Organisms [8], Drugs [9], Animal Care [10], Cattle [11], Research [12], United States Of America (US) [13]
Centre for Science and Environment
National Knowledge Commission Government of India

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Source URL: http://admin.indiaenvironmentportal.org.in/feature-article/diverse-antibiotic-resistance-genes-dairy-cow-manure

Links:
[1] http://admin.indiaenvironmentportal.org.in/feature-article/diverse-antibiotic-resistance-genes-dairy-cow-manure
[2] http://mbio.asm.org/content/5/2/e01017-13
[3] http://admin.indiaenvironmentportal.org.in/category/author/fabienne-wichmann
[4] http://admin.indiaenvironmentportal.org.in/category/author/nikolina-udikovic-kolic
[5] http://admin.indiaenvironmentportal.org.in/category/author/sheila-andrew-et-al
[6] http://admin.indiaenvironmentportal.org.in/category/journal/mbio
[7] http://admin.indiaenvironmentportal.org.in/category/thesaurus/animal-dung
[8] http://admin.indiaenvironmentportal.org.in/category/thesaurus/micro-organisms
[9] http://admin.indiaenvironmentportal.org.in/category/thesaurus/drugs
[10] http://admin.indiaenvironmentportal.org.in/category/thesaurus/animal-care
[11] http://admin.indiaenvironmentportal.org.in/category/thesaurus/cattle
[12] http://admin.indiaenvironmentportal.org.in/category/thesaurus/research
[13] http://admin.indiaenvironmentportal.org.in/category/thesaurus/united-states-america-us