The present study has been carried out to assess groundwater quality in parts of Hindon–Yamuna interfluve region of western Uttar Pradesh. Fifty-five groundwater samples were collected from hand pumps in post-monsoon 2005 and pre-monsoon 2006 period, respectively, covering an area of about 1,345 km2. Physical and chemical parameters of groundwater such as electrical conductivity, pH, total dissolved solid, Na, K, Ca, Mg, HCO3, Cl, and SO4 were determined.