The recent increase in nanoparticle (P25 TiO2 NPs) usage has led to concerns regarding their potential implications on environment and human health. The food chain is the central pathway for nanoparticle transfer from lower to high trophic level organisms. The current study relies on the investigation of toxicity and trophic transfer potential of TiO2 NPs from marine algae Dunaliella salina to marine crustacean Artemia salina.