Domiasiat (25°30′N 91°30′E) located in the west Khasi hill district of Meghalaya in northeast India is one of the largest sandstone-type uranium (U) ore deposit in India containing 9.22 million tonnes of ore reserves with an average ore grade of around 0.1 % U3O8. This geographically distinct U deposit of Domiasiat is un-mined and harbours diverse group of bacteria surviving the stressful environmental conditions prevalent in the ore deposit. Studies show that the diverse bacteria belonged to 10 different bacterial groups with occurrence of some previously uncharacterized bacteria.