This study presents biogas production from fruit wastes at ambient temperature using anaerobic batch digester (500 ml). Co-digestion of fruit wastes were carried out with rice bran and cow dung. Biogas production from different samples was as follows: Sample A (fruit waste), 363; sample B (fruit waste, 75% + cow dung, 25%), 405; sample C (fruit waste, 75% + rice bran, 25%), 315; and sample D (fruit waste, 50% + rice bran, 25% + cow dung, 25%), 381 ml. From chromatographic studies, sample B has maximum methane content (80%) and sample C has maximum carbon-di-oxide content (50%). Cow dung influences digestion of fruit wastes and showed highest yield (405 mg) of biogas production.