In an attempt to reduce environmental loading during ethanol production from cellulosic plant biomass, we have previously proposed an on-site solid state fermentation (SSF) method for producing ethanol from whole crops, which at the same time provides cattle feed without producing wastes. During the ensiling of freshly harvested plant biomass with cellulase and glucoamylase, the added yeast and lactic acid bacteria induced simultaneous saccharification and production of ethanol and lactic acid in hermetically sealed containers on-farm. In a previous study, laboratory-scale SSF (using 250 g of fresh rice crop biomass) yielded 16.9 weight % ethanol in dry matter (DM) after 20 days of incubation. In this study, the fermentation volume was scaled up to a normal-sized round bale and the fermentation process (ethanol concentrations of the products) was monitored. The ethanol produced was recovered and the recovery efficiency was evaluated.

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