Property changes associated with differences in chemical structure between vegetable oil and petroleum based diesel fuel may ask for change in the engine operating parameters such as injection timing, injection pressure etc. These operating parameter changes can cause difference in performance and exhaust emissions than the optimized settings chosen by the engine manufacturer. In the present work, an experimental set was developed to investigate the effect of injection timing and injection pressure in Kirloskar AV1 diesel engine operated on heated Karanj-diesel blend.