Chemical effluents released from factories that came up on the outskirts of Adilabad town into the tanks of the surrounding villages caused the death of 40 quintals of fish in the last two days. Villagers and fishermen of Chanda (T) village of Adilabad staged a protest on Wednesday along with dead fish brought in gunny bags alleging negligence on the part of officials concerned in taking action against managements of private factories that released harmful effluents.