The present investigation deals with the chemical characteristics/composition of the natural waters of the Nilgiri Biosphere Reserve (NBR), located within Kerala and Karnataka states respectively. Waters of both localities differ significantly in their chemical characteristics and composition. The waters of NBR - Kerala are bereft of dissolved minerals in their per unit volume, irrespective of their sources and location. In contrast to above, the waters of Karnataka have sufficient dissolved minerals in their per unit volume. The present study pertinently suggests that to compensate the mineral deficiency especially in carnivores, the salt-licks having sufficient minerals may be placed in the entire biosphere reserve under Kerala and within Karnataka, it may be placed within those area which are not covered by artificial tanks.