Dyes present in the effluent of textile industries are recalcitrant molecules difficult to be degraded biologically. In this study we used Pseudomonas fluorescens to degrade Direct Orange - 102 dye. Pseudomonas fluorescens NCIM 2100 was obtained from National Chemical Laboratory, Pune, India, that was adapted to grow on Direct Orange - 102. The dye was subjected to degradation by the bacterium and its metabolic products were identified by UV, 1HNMR and IR spectrophotometry. The dye was first broken down into 3, 7- diamino- 4 hydroxy - naphthalene - 2 sulfonic acid sodium salt. This compound is further degraded into 7-amino -3, 4- dihydroxy - naphthalene- 2- sulfonic acid sodium salt or 3- amino-4-7- dihydroxy-naphthalene -2 sulfonic acid sodim salt or 1,3,4,5,6,7,8 - heptahydroxy naphthalene - 2 - sulfonic acid sodium salt. These breakdown compounds were non - toxic in nature. Therefore, Pseudo-monas fluorescens can be used for bioremediation of textile effluent containing Direct Orange-102 dye.