Azoxystrobin residue was determined in mango fruits using high performance liquid chromatography (HPLC). Residues of azoxystrobin were extracted with acetonitrile and purified by both liquid liquid extraction (LLE) and solid phase extraction (SPE) clean up. The HPLC analysis was carried out on ODS2 column with acetonitrile: water (80:20 v/v) as the mobile phase with UV