The present study focuses on the transformations of a typical Mediterranean agroforestry landscape of southern Italy (high Agri Valley – Basilicata region) that occurred over 24 years. In this period, the valuable agricultural and natural areas that compose such a landscape were subjected to intensive industry-related activities linked to the exploitation of the largest European onshore oil reservoir. Landsat imagery acquired in 1985 and 2009 were used to detect changes in forest areas and major land use trajectories.