This study presents ethanol production from mahula flowers in submerged fermentation (SmF) using immobilized cells of Saccharomyces cerevisiae (CTCRI strain) and Zymomonas mobilis (MTCC 92) in calcium alginate as beads. Maximum ethanol concentrations were 154.5 and 134.55 g kg-1 flowers using immobilized cells of S. cerevisiae and Z. mobilis, respectively. Immobilized cells of S. cerevisiae in calcium alginate beads were more effective (14.83% more yield) for ethanol production than immobilized cells of Z. mobilis.