Central Water Commission has been playing a major role in the water quality monitoring of river water since year 1963 and at present, is observing water quality at 658 key locations (625 on HO network and 33 WQSS) covering major river basins of India. As per Guidelines of Water Quality Monitoring, 2017 pesticide analysis of river water samples also necessary and frequency of pesticide analysis is given as once in a year during pre-monsoon. This book titled as “Analysis of pesticides in Water Samples” is to provide brief knowledge about pesticides and its classification, fate and effects of pesticides, sample collection, containers, sample storage, preservation and transportation, methods involving for the pesticides analysis.