Cholera, the most dreadful of all diarrhoeal diseases, is an acute intestinal infection, caused by Vibrio cholerae, which afflicts 3 to 5 million of people and causes 0.1 million deaths every year . Cholera outbreaks have been infrequently reported from
developed countries and often reported from various parts of developing, and underdeveloped countries mainly owing to poor sanitation of potable water. In India sporadic cases of acute watery diarrhoea occur throughout the year, especially in rural areas.