India’s phenomenal economic growth over the last decade has been accompanied by a much-needed expansion and improvement in transport and other infrastructure networks. While there are legally mandated assessments of the potential ecological impacts of such infrastructure projects prior to implementation, rarely are there post-implementation assessments of their real ecological impacts. In this communication, we present results of a preliminary study examining the impact of vehicular traffic on the usage of road edges by large mammals along a highway passing through Nagarahole Tiger Reserve, southern India