The immediate and most common response by the different organs of a plant to water stress is decrease in turgor. This may be partially or fully adjusted by
accumulation of solutes. In the present study sorghum pollen grains were subjected to in vitro osmotic stress using polyethylene glycol (PEG). The change in size and shape of the pollen grains under osmotic stress
was considered as a measure of osmotic adjustment (OA). The in vitro pollen response to osmotic stress with and without osmolyte and genotypic response to pollen OA vis-à-vis leaf OA was analysed in kharif and rabi sorghum genotypes.