Bt cauliflower is one of the important genetically modified
(GM) crops approved for Biosafety Research Level 1 field trials in India. Towards developing reliable qualitative and quantitative PCR methods for detecting and monitoring GM crops, in the present study, molecular detection of cry1Ac gene CaMV 35S
promoter and an endogenous gene in Bt cauliflower was carried out by simplex and multiplex PCR. Furthermore, validation of S-locus receptor kinase (SRK) as the endogenous reference gene for the Brassicaceae family using simplex PCR has also been undertaken.