This report represents the proceedings of the FAO international technical conference dedicated to Agricultural Biotechnologies in Developing Countries (ABDC-10) that took place in Guadalajara, Mexico on 1-4 March 2010. A major objective of the conference was to take stock of the application of biotechnologies across the different food and agricultural sectors in developing countries, in order to learn from the past and to identify options for the future to face the challenges of food insecurity, climate change and natural resource degradation.