This report provides comprehensive information about the glacial lakes of five major river basins of the Hindu Kush Himalaya (HKH) — Amu Darya, Indus, Ganges, Brahmaputra, and Irrawaddy, including Mansarovar Interior Basin — representing the year 2005, which helps to fill the data gap of glacial lakes information in the region. This is the first comprehensive knowledge upon the distribution of glacial lakes for the HKH providing baseline data for further investigation of glacial lakes, GLOF hazards and risk assessment, and mitigation measures.