This practical guide for cities is highlighting how a focus on ecosystem services and their valuation can create direct benefits for urban areas and can be performed even with limited resources. The TEEB Manual is a practical resource, outlining the step-wise approach introduced in the TEEB report with in-depth real-life case examples from cities around the world that have employed this approach. This manual aims to inspire cities to start thinking about ecosystem services and how a focus on their valuation can be useful at the local situation, thus creating sustainable and resilient cities.