Trace greenhouse gases are a fundamentally important component of Earth’s global climate system sensitive to global change. However, their concentration in the pre-Pleistocene atmosphere during past warm greenhouse climates is highly uncertain because we lack suitable geochemical or biological proxies.

Destruction of the Earth's ozone shield due to the release of hydrogen sulphide and methane has been suggested as a cause of mass extinctions during periods of ocean anoxia over the past two billion years. This mechanism does not explain the end-Permian mass extinction, according to simulations with a two-dimensional atmospheric chemistry-transport model, which show that the ozone shield remains intact even with massive releases of hydrogen sulphide and methane.